Objective  The objective of this study is to research the dynamic changes and correlation of rhizosphere soil enzyme activities and microbial community structure in different growth stages of Angelica sinensis, so as to provide scientific basis for healthy planting and soil improvement of Angelica sinensis.

  Method  Taking the rhizosphere soil of A. sinensis in Gansu Province as the research object, the activities of five primary metabolic enzymes and the changes of phylum and genus levels of bacterial and fungal communities by high-throughput sequencing were studied in five growth periods.

  Result  It was found that the richness and evenness of bacterial community in rhizosphere soil of A. sinensis did not change with growth periods, but the fungal community changed significantly. The community structure of bacteria and fungi in the late growth stages were significantly separated from that in the early and middle stages, which were mainly caused by the significant changes in the abundance of some phylum and genus. The relationship between bacteria tends to be mutually beneficial symbiotic, but between bacteria and fungi tends to be negative correlation. The invertase activities of rhizosphere soil were the lowest and urease activities were the highest at bolting stage. The correlation analysis showed that the effect of bacteria on enzyme activities was higher than that of fungi. Invertase was significantly positively correlated with Flavobacterium, urease was significantly positively correlated with Bacteroides and Chaetomium, and was significantly negatively correlated with Massilia, Sphingobium, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium and Novosphingobium. Acid phosphatase, amylase and catalase remained stable in different periods. Sphingomonas, Bacteroides, MND1, Gemmatimonas and Lysobacter played an important role in maintaining their activities.

  Conclusion  This study reflects the dynamic balance of bacterial-fungal community in A. sinensis rhizosphere soil during its growth and development. The growth cycle fluctuation and correlation of enzymes and bacteria reflect some enzymes and bacteria those may be related to A. sinensis bolting, and the relevant bacteria that maintain enzyme stability. This study provides a scientific reference for the screening and functional verification of growth promoting bacteria of A. sinensis in the next step.