王庆峰, 周德平, 褚长彬, 赵　峥, 杨乾罡, 吴淑杭. 炭疽病发病草莓与健康草莓根际细菌群落结构及功能差异[J]. 土壤通报, 2022, 53(6): 1404 − 1412. DOI: 10.19336/j.cnki.trtb.2021110906
引用本文: 王庆峰, 周德平, 褚长彬, 赵　峥, 杨乾罡, 吴淑杭. 炭疽病发病草莓与健康草莓根际细菌群落结构及功能差异[J]. 土壤通报, 2022, 53(6): 1404 − 1412. DOI: 10.19336/j.cnki.trtb.2021110906
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WANG Qing-feng, ZHOU De-ping, CHU Chang-bin, ZHAO Zheng, YANG Qian-gang, WU Shu-hang. Differences in Structure and Function of Rhizosphere Bacterial Communities between Anthracnose-infected and Healthy Strawberries[J]. Chinese Journal of Soil Science, 2022, 53(6): 1404 − 1412. DOI: 10.19336/j.cnki.trtb.2021110906
Citation: WANG Qing-feng, ZHOU De-ping, CHU Chang-bin, ZHAO Zheng, YANG Qian-gang, WU Shu-hang. Differences in Structure and Function of Rhizosphere Bacterial Communities between Anthracnose-infected and Healthy Strawberries[J]. Chinese Journal of Soil Science, 2022, 53(6): 1404 − 1412. DOI: 10.19336/j.cnki.trtb.2021110906
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炭疽病发病草莓与健康草莓根际细菌群落结构及功能差异

Differences in Structure and Function of Rhizosphere Bacterial Communities between Anthracnose-infected and Healthy Strawberries

    摘要
  • 摘要:
      目的   比较研究炭疽病不同发病程度草莓根际微生物群落结构并开展基因功能预测分析，为指导农田管理及筛选草莓炭疽病拮抗菌提供理论依据和参考。
      方法  采集设施栽培大棚中健康和不同发病程度的草莓植株根际土壤，利用Illumina Miseq测序比较分析不同发病程度对草莓根际细菌多样性，根际细菌门、属水平群落组成特征的影响。
      结果  发病植株根际细菌多样性显著低于健康植株，其中轻度发病草莓根际细菌丰富度ACE降低了7.2%，而中度发病草莓根际细菌丰富度降低了11%。草莓根际土壤的优势菌群为变形菌门、放线菌门和拟杆菌门，不同发病程度的草莓根际土壤的细菌群落结构发生了显著改变，其中发病草莓显著提高了根际土壤中芽孢菌属和亚栖热菌属的相对丰度，而降低了与促生功能相关的黄杆菌属和伯克氏菌属的相对丰度。PICRUSt2分析表明，炭疽病显著提高草莓根际细菌的细胞交流、细胞传递和分解，转录以及循环等功能，而降低了脂质代谢和消化功能。
      结论  本研究表明炭疽病显著降低草莓根际细菌多样性，并改变细菌群落结构，降低了促进草莓营养吸收细菌种类的相对丰度，改变了根际细菌功能特征，研究结果为指导草莓生产管理和筛选抗病微生物提供参考。

     

    Abstract:
      Objective  The aim of this study is to compare the rhizosphere microbial community structure and function of strawberry among difference degrees of anthracnose, which is crucial for guiding farmland management and screening useful strawberry anthracnose antagonistic resource.
      Method  The strawberry rhizosphere soil among difference degrees of anthracnose in the same field was collected in a greenhouse for studying the rhizosphere bacterial diversity and richness at phylum and genus levels by using Illumina sequencing and PICRUSt2.
      Result  The results showed that anthracnose decreased rhizosphere bacterial diversity, with the mild degrees of anthracnose decreased 7.2% (ACE) and the middle decreased 11%. The phyla Proteobacteria, Actinobacteria and Bacteroidetes dominated in all strawberry rhizosphere soil. The different degrees of anthracnose changed rhizosphere soil bacterial composition, with an increase of Bacillus. spp and Meiothermus. Spp, while a decreased of Flavobacterium and Burkholderia. The PICRUSt2 analysis showed that the occurrence of anthracnose disease increased cell communication, transport and catabolism, transcription, but decreased lipid metabolism and digestive system.
      Conclusion  The strawberry anthracnose change rhizosphere bacterial composition, functional traits and decrease diversity. In addition, the strawberry anthracnose also decreases the relative abundance of bacterial taxa and enhances plant nutrient absorption.

     

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