刘 威, 朱 尧, 吕自敏, 张永瑞, 郭桂义. 杉木林转茶园和草地后土壤微生物群落结构变化[J]. 土壤通报, 2024, 55(4): 1041 − 1049. DOI: 10.19336/j.cnki.trtb.2023052501
引用本文: 刘 威, 朱 尧, 吕自敏, 张永瑞, 郭桂义. 杉木林转茶园和草地后土壤微生物群落结构变化[J]. 土壤通报, 2024, 55(4): 1041 − 1049. DOI: 10.19336/j.cnki.trtb.2023052501
LIU Wei, ZHU Yao, LÜ Zi-min, ZHANG Yong-rui, GUO Gui-yi. Soil Microbial Community Structure Changes After Conversion of Chinese Fir Forest to Tea Garden and Grassland[J]. Chinese Journal of Soil Science, 2024, 55(4): 1041 − 1049. DOI: 10.19336/j.cnki.trtb.2023052501
Citation: LIU Wei, ZHU Yao, LÜ Zi-min, ZHANG Yong-rui, GUO Gui-yi. Soil Microbial Community Structure Changes After Conversion of Chinese Fir Forest to Tea Garden and Grassland[J]. Chinese Journal of Soil Science, 2024, 55(4): 1041 − 1049. DOI: 10.19336/j.cnki.trtb.2023052501

杉木林转茶园和草地后土壤微生物群落结构变化

Soil Microbial Community Structure Changes After Conversion of Chinese Fir Forest to Tea Garden and Grassland

  • 摘要:
    目的 探究杉木林转变为茶园和草地后土壤微生物群落结构及多样性特点,并分析土壤环境因子与微生物群落结构的相关性。
    方法 采用Illumina Miseq高通量测序技术,研究了杉木林、茶园和草地等3种植被类型土壤真菌、细菌群落结构变化,并分析土壤化学因子对土壤真菌、细菌群落结构的影响。
    结果 杉木林改种茶园后,茶园土壤子囊菌门相对丰度提高33.55%,茶园土壤担子菌门相对丰度下降了34.93%;杉木林改草地后土壤子囊菌门相对丰度下降33.86%,担子菌门相对丰度提高19.41%;茶园和草地土壤细菌厚壁菌门相对丰度分别降低74.54%和82.72%,而变形菌门相对丰度分别提高23.33%和31.59%,酸杆菌门相对丰度分别提高35.85%和72.52%。土壤真菌群落多样性及丰富度均为杉木林显著高于茶园和草地;杉木林土壤细菌丰富度显著高于茶园和草地。RDA相关性分析结果表明,细菌群落结构受土壤环境因子的影响大于真菌,土壤pH是影响细菌和真菌群落最主要的环境因子,其次是铵态氮和碱解氮。
    结论 杉木林转茶园和草地后,真菌物种多样性及相对丰度均降低,细菌物种多样性增加,相对丰度有所降低。由杉木林转化而来的茶园和草地土壤中细菌、真菌群落结构差异较大。

     

    Abstract:
    Objective The aims were investigate the characteristics of soil microbial community structure and diversity in Chinese fir forest, tea garden and grassland, and to analyze the correlation between soil environmental factors and microbial community structure.
    Method Soil samples were collected from Chinese fir forest, tea garden, and grassland. The Illumina Miseq high-throughput sequencing technology was used to analyze the characteristics of soil fungal and bacterial communities in these three vegetation types. The soil chemical factors were also measured to determine their effects on soil fungal and bacterial communities.
    Results After conversion of Chinese fir forest to tea garden, the relative abundance of Ascomycota in tea garden soil increased by 33.55%, while the relative abundance of Basidiomycota decreased by 34.93%. After conversion of Chinese fir forest to grassland, the relative abundance of Ascomycota in grassland soil decreased by 33.86%, while the relative abundance of Basidiomycota increased by 19.41%. The relative abundance of Firmicutes decreased by 74.54% and 82.72% in tea garden and grassland soil, respectively, while the relative abundance of Proteobacteria increased by 23.33% and 31.59%, respectively. The relative abundance of Acidobacteria increased by 35.85% and 72.52% in tea garden and grassland soil, respectively. The diversity and richness of soil fungal communities in Chinese fir forests were significantly higher than those in tea gardens and grasslands, while the richness of soil bacteria in Chinese fir forests was significantly higher than those in tea gardens and grasslands. The relative abundance of soil bacteria was significantly higher in Chinese fir forest than in tea garden and grassland. Redundancy analysis showed that the soil bacterial community structure was more influenced by soil environmental factors than the fungal community structure. pH was the most important environmental factor affecting both bacterial and fungal communities, followed by ammonium nitrogen and effective nitrogen.
    Conclusion After conversion of Chinese fir forest to tea garden and grassland, the diversity and relative abundance of fungal species decreased, while the diversity of bacterial species increased but their relative abundance decreased. There are significant differences in the structure of bacterial and fungal communities between tea garden and grassland soils derived from Chinese fir forest.

     

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