Objective The Pisha sandstone area of Inner Mongolia is one of the most serious areas of soil and water loss in the Loess Plateau and even the world. Given the current problems of ecological function degradation and serious soil and water loss in the Pisha sandstone area, the microbial characteristics of rhizosphere soil under Hippophae rhamnoides plantation after stumping in the Pisha sandstone area were mastered, and the effect of stumping on the microbial community structure of rhizosphere soil under Hippophae rhamnoides was clarified.

Method In this study, Hippophae rhamnoides plantation, which has entered a recession period in this area, was used as the research object. Illumina MiSeq high-throughput sequencing method was used to analyze the microbial community diversity and community structure of the rhizosphere soil of Hippophae rhamnoides under stumping ( stubble 0 cm, stubble 10 cm, 15 cm, 20 cm ) and non-stubble conditions.

Result The results showed that:①The physical and chemical properties of rhizosphere soil showed that the 15 cm stubble treatment was superior to other stubble treatments, and the pH of rhizosphere soil did not change significantly under each stubble treatment. ②The specific amplicon sequence variation ( ASV ) of 15 cm stubble treatment was the most. The alpha diversity of bacterial community was the highest in 0-10 cm soil layer, the highest in 15 cm soil layer of flat stubble treatment group, and the flat stubble treatment group was higher than that of non-flat stubble treatment. The composition of 0-10 cm rhizosphere soil depth was similar, the composition of 20-30 cm rhizosphere soil depth was similar, the composition of 10-20 cm rhizosphere soil depth was not similar, and the distribution of 15 cm in the stubble treatment group was relatively independent.③A total of 37 phyla, 116 classes, 227 orders, 356 families, 634 genera and 697 species of bacteria were detected in the rhizosphere soil samples of the four Hippophae rhamnoides stubble patterns and the control group. The relative abundance of 0-10 cm soil layer was the highest at the genus level, and the relative abundance of 15 cm stubble treatment was the highest at the genus level. ④The correlation analysis between the physical and chemical properties of rhizosphere soil and the abundance of bacterial dominant phylum showed that the available phosphorus in rhizosphere soil had a very significant correlation with most bacterial dominant phylum.

Conclusion In short, when the stubble height of Hippophae rhamnoides is 15 cm, it is the best. Stubble is beneficial to the restoration of rhizosphere soil under Hippophae rhamnoides forest, which indirectly reflects that stubble is beneficial to the renewal and rejuvenation of Hippophae rhamnoides. The research results can provide a theoretical basis for the regeneration and ecological restoration of Hippophae rhamnoides plantation in Pisha sandstone area.