Objective The aims were to reveal the effects of the ratio of green manure, microbial agents, and trace element fertilizers on the growth of strawberry plants and the improvement of strawberry culture substrates.

Method Three types of fertilization treatments were used: granular green fertilizer (G), granular bio green fertilizer (green fertilizer + Bacillus subtilis + Trichoderma longum, GJM), granular trace element green fertilizer (green fertilizer + trace element fertilizer, GW). Two cultivation substrates were farmland soil and peat soil. The effects of different fertilization ratios on the growth of strawberry plants and the nutrient contents of the two culture substrates were studied.

Result It was found that regardless of whether farmland soil or peat soil was used as the cultivation substrate, the agronomic traits of strawberry plants treated with the three fertilizers were significantly higher than those of the control treatment throughout the entire growth period. In different treatments using peat soil as the culture medium, GJM treatment increased the aboveground dry weight by 310.73%, 155.52% and 60.74%, respectively, while the underground dry weight increased by 634.37%, 149.72% and 27.19%, respectively. Different fertilization treatments could all increase the nutrient contents of farmland soil and peat soil, and each fertilization treatment could significantly increase the available nutrient contents of the substrate compared to CK and G treatments. Among them, the GJM treatment had the highest content of soil available nitrogen, significantly increasing by 34.41% and 14.68% compared to CK and G treatments. And its promoting effect on hydrolyzed nitrogen content was significantly higher than that of GW treatment. GW treatment increased the contents of available phosphorus and potassium in farmland soil and peat soil the most, followed by GJM treatment, but there was no significant difference between the two treatments.

Conclusion The combined application of green manure, microbial agents, and trace elements (GJM) has a significant promoting effect on the agronomic traits of strawberry growth and a significant improvement in the nutrients of the culture substrate.